Micro and Nanofluidics Our research focuses on the development of microfabricated instrumentation and application in various chemical and biochemical problems. Microfluidic Separations Cell Based Studies Fundamental Characteristics Nanofluidic Sensing Microfluidics High-Efficiency Electrophoretic Analysis of N-Glycans. N-Glycan profiles of glycoproteins derived from human serum analyzed by microfluidic capillary electrophoresis (CE) and capillary electrophoresis-electrospray ionization-mass spectrometry (CE-MS). Nanopores Design and characterization of in-plane and out-of-plane nanofluidic devices. An SEM image (left) and AFM image (right) of a 20° tapered nanofunnel formed by electron beam lithography. Bacteria Programmable, pneumatically actuated microfluidic device with an integrated nanochannel array to track development of individual bacteria. Tracking of live cells by video microscopy. Sensing Characterize the assembly of hepatitis B virus core protein dimers into T = 3 and T = 4 icosahedral capsid by resistive-pulse sensing. Improve the practical-size resolution by multicycle resistive-pulse sensing with four pores in series.